Abbott i-Stat Activated Clotting Time, Celite - Point of Care Testing

Abbrev Code:ACTC   
Order Code:POC200Order Name:Activated Clotting Time POCT
Synonyms:ACT, Activated Clotting Time
Methodology:Activated whole blood clotting time
CPT Codes: 85347 x1
Turnaround Time:Performed daily; results are available immediately.
Special Instructions:Call the lab to schedule/order laboratory performed testing at the bedside

Collection Instructions

Specimen:Whole Blood
Optimal Volume:0.2 mL
Minimum\Peds Volume:0.04 mL
Container:See Collection Instructions, Plain syringe
Alternate Containers: ACT cartridge available from laboratory
Collection Instructions:Venipuncture Collection: Use two plain syringes to minimize thromboplastin contamination. Collect 2 mL into the first syringe and discard, into the second syringe collect 0.2 mL to be used for testing. Do not use heparinized syringes. Catheter Collection: Flush anticoagulated catheter with 5 mL of saline. Collect 5-10 mL and discard, into a second plain syringe collect 0.2 mL to be used for testing.
Causes for Rejection:Extraneous heparin present in the sample; heparinized syringes; samples not collected immediately prior to testing.
Contraindications:Testing should be performed immediately. If there is a delay in testing or a repeat measurement is needed, a fresh sample must be collected.

Processing and Shipping

Stability:Test immediately. For questions, contact the laboratory point of care team at your facility.
Test Performed at or Referral Lab Point of Care  (UMMC-West Bank, UMMC-East Bank, Ridges, Southdale)


Reference Range: 75-150 seconds
Therapeutic Range:Dependent on clinical procedure.
Sheath Pull Guidelines:
  • If value is < 180 seconds, no further testing is required. The laboratory places orders for follow-up testing if the result is >180 seconds.
  • If value is greater than 180, repeat ACT in 60 minutes.
  • If value changed by less than 6 seconds, recommend a heparin 10a level. Rare patients may have a baseline ACT above 180 seconds and heparin 10a level of less than 0.10 IU/mL, indicating little or no heparin is present in the sample.

Testing Guidelines for PRISMA Patients:
  • 160-180 seconds for normal heparin.
  • 140-150 for minimal heparin
Use:Monitor a patient's anticoagulation state during high dose heparin administration.

Click HERE to Report test errors or omissions.
*If no email program is associated with this computer, please contact: for TestID: 6467"