Heparin Induced Thrombocytopenia, Reflex to Serotonin Release Assay


Abbrev Code:HITR   
Order Code:LAB7075Order Name:Heparin Induced Thrombocytopenia, Reflex
Synonyms:HIT; HIT Reflex; HITSC; HITET
Methodology:Immunoassay; Serotonin release assay if reflexed.
CPT Codes: 86022 x1, 86022 x1, 86022 x1, 86022 x1
Turnaround Time:Mon-Sat: If specimen is received by 1 pm in the Special Coag lab, the test will be done that day. For urgent testing after hours call 612-273-5551 to contact the tech on call.
Associated Links:

Specimen Collection and Processing for Coagulation Specimens


Collection Instructions

Specimen:Blood
Optimal Volume:Blue Na Citrate: 2.7 mL (in 3 mL tube) and Red or gold (gel) or Red (no gel): 10 mL.
Minimum\Peds Volume:Blue Na Citrate: 1.8 mL (in 2 mL tube) and Red or gold (gel) or Red (no gel): 2.2 mL.
Container:Red or gold (gel) AND Blue Na Citrate, 3.2% - fill to fill line on tube
Alternate Containers: Red (no gel) AND Blue Na Citrate, 3.2% - fill to fill line on tube
Collection Instructions:Collect both Blue Na Citrate and a Red or Gold (gel), or a Blue Na Citrate and a red (no gel) tube.
See link to Specimen Collection and Processing for Coagulation Specimens.
Causes for Rejection:Underfilled tubes, clotted specimens, specimens not received or processed and frozen within 4 hours of collection. Samples with excessive hemolysis, icterus or lipemia should not be used.


Processing and Shipping

Specimen Processing:
  • Process for Platelet Poor Plasma within 4 hours of collection.
  • Aliquot plasma, place clear tape around label, and freeze at -20C or on dry ice within 4 hours of collection.
  • If multiple tests are collected on 1 blue top, split each blue top into 2 aliquots. Minimum 0.5 mL per tube.
  • A maximum of 6 aliquots are needed per collection.
     
See link to Specimen Collection and Processing for Coagulation Specimens.
Shipping Instructions:
  • Ensure specimen is frozen before shipping.
  • Ship on dry ice, sample must remain frozen during shipping.
     
See link to Specimen Collection and Processing for Coagulation Specimens.
Test Performed at or Referral Lab UM Special Coag (K)  (UMMC East Bank)


Interpretive

Critical Range:Positive
Reference Range:Negative
Use:HemosIL HIT-Ab (PF4-H) is a qualitative, fully automated, latex enhanced immunoassay for the detection of anti-platelet factor 4/heparin (PF4/H) antibodies. The positive or negative result aids in determining the risk for heparin induced thrombocytopenia (HIT) when used in conjunction with other laboratory and clinical findings. Anti-PF4/Heparin antibodies are commonly found in patients with HIT. For use in adult population suspected of HIT. Not for use in isolation to exclude HIT,


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