Heparin Induced Thrombocytopenia, Reflex to Serotonin Release Assay
| Sunquest Code: | HITR | ||
| Epic Code: | LAB7075 | Epic Name: | Heparin Induced Thrombocytopenia, Reflex |
| Synonyms: | HIT; HIT Reflex; HITSC; HITET | ||
| Methodology: | Immunoassay; Serotonin release assay if reflexed. | ||
| Turnaround Time: | Mon-Sat: If specimen is received by 1 pm in the Special Coag lab, the test will be done that day. For urgent testing after hours call 612-273-5551 to contact the tech on call. | ||
| Associated Links: | Specimen Collection and Processing for Coagulation Specimens |
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Collection Instructions
| Specimen: | Blood |
| Optimal Volume: | Blue Na Citrate: 2.7 mL (in 3 mL tube) and Red or gold (gel) or Red (no gel): 10 mL. |
| Minimum\Peds Volume: | Blue Na Citrate: 1.8 mL (in 2 mL tube) and Red or gold (gel) or Red (no gel): 2.2 mL. |
| Container: | Red or gold (gel) AND Blue Na Citrate, 3.2% - fill to fill line on tube [Alternate Containers: Red (no gel) AND Blue Na Citrate, 3.2% - fill to fill line on tube] |
| Collection Instructions: | Collect both Blue Na Citrate and a Red or Gold (gel), or a Blue Na Citrate and a red (no gel) tube. See link to Specimen Collection and Processing for Coagulation Specimens. |
| Causes for Rejection: | Underfilled tubes, clotted specimens, specimens not received or processed and frozen within 4 hours of collection. Samples with excessive hemolysis, icterus or lipemia should not be used. |
Processing and Shipping
| Specimen Processing: | See link to Specimen Collection and Processing for Coagulation Specimens. |
| Shipping Instructions: | See link to Specimen Collection and Processing for Coagulation Specimens. |
| Test Performed at or Referral Lab | Special Coagulation (UMMC-East Bank) |
Interpretive
| Critical Range: | Positive |
| Reference Range: | Negative |
| Use: | HemosIL HIT-Ab (PF4-H) is a qualitative, fully automated, latex enhanced immunoassay for the detection of anti-platelet factor 4/heparin (PF4/H) antibodies. The positive or negative result aids in determining the risk for heparin induced thrombocytopenia (HIT) when used in conjunction with other laboratory and clinical findings. Anti-PF4/Heparin antibodies are commonly found in patients with HIT. For use in adult population suspected of HIT. Not for use in isolation to exclude HIT, |
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