CD34 Absolute Count

Abbrev Code:34ABS   
Order Code:LAB8001Order Name:CD34 Absolute Count
Synonyms:CD34 Hematopoietic Stem Cell Count
Methodology:Single platform multi-color immunofluorescence and flow cytometry using ISHAGE guidelines
CPT Codes: 86367 x1
Test Includes:Enumeration of hematopoietic progenitor stem cells using a combination of CD45/CD34 monoclonal antibodies. Viability of CD34 positive cells is also assessed.
Turnaround Time:24 hours
Special Instructions:Testing is performed Mon-Fri 08:00-18:00.  If testing is needed outside of scheduled hours, contact the Flow Cytometry lab at 612-273-5248. Provide diagnosis, age, collection date and time, specimen type, and any product information. Samples should be sent STAT. Samples are received in the Core lab when the Flow Cytometry lab is not staffed

This test was developed and its performance characteristics determined by University of Minnesota Medical Center, Fairview Clinical Laboratories. It has not been cleared or approved by the US Food and Drug Administration. FDA does not require this test to go through premarket FDA review. This test is used for clinical purposes and should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.

Collection Instructions

Specimen:Blood, peripheral blood progenitor cell collection, processed bone marrow.
Optimal Volume:3 mL blood; 1 mL peripheral blood progenitor cell collection; 10 x 106 processed bone marrow cells.
Minimum\Peds Volume:1 mL blood; 0.5 mL peripheral blood progenitor cell collection.
Container:Purple (EDTA)
Collection Instructions:
Collect all  specimens aseptically:
  • Blood: Collect in purple top (EDTA) tube, mix well.
  • Peripheral Blood Progenitor Cell Collection: Collect in purple top (EDTA) tube, mix well.
  • Processed Bone Marrow: Place cells in sterile container.

    The sending location should contact IFC at 612-273-5248 prior to sending specimen.
Causes for Rejection:Improper specimen collection or handling; clotted specimen; specimen received more than 24 hours after collection.

Processing and Shipping

Specimen Processing:Do not centrifuge. Store in refrigerator. Contact IFC Laboratory prior to sending specimen. See comments.
Shipping Instructions:Ship STAT at room temperature. Must arrive within 24 hours.
Test Performed at or Referral Lab UM Flow Cytometry (W)  (UMMC East Bank)


Use:Flow cytometric evaluation of CD34+ cell level is necessary for measuring the hematopoietic progenitor cells in dose requirement protocols of stem cell transplantation. Quantification of CD34+ cells is also used during mobilization of hematopoietic cells and leukophoresis procedures. The total number of CD34+ cells collected during leukophoresis can be estimated.

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