Diffuse Large B Cell Lymphoma (DLBCL) NGS Panel

Sunquest Code:DLBCLCoPath Code:DLBCL
Epic Code:LAB8068Epic Name:Diffuse Large B Cell Lymphoma (DLBCL) NGS Panel
Methodology:Next generation sequencing
Test Includes:CARD11, CD79A, CD79B, CREBBP, EZH2, MYD88, NOTCH2, TET2, TP53, BTK
Turnaround Time:Performed Mon-Fri; results are reported within 10-14 days.

Collection Instructions

Specimen:Blood, bone marrow
Optimal Volume:10 mL blood; 3 mL bone marrow
Minimum\Peds Volume:3 mL blood; 1 mL bone marrow
Container:Yellow (ACD, Solution A) tube available from laboratory (Purple (EDTA))
Collection Instructions:Bone marrow clot acceptable for testing.
Causes for Rejection:Whole blood: frozen or clotted specimen, incorrect anticoagulant, specimen more than 5 days old. All testing will be canceled if engraftment testing is 100% donor engrafted in consultation with clinician.
Shared blood or bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers. Follow up samples for the hematology panels will be tested for all gene(s) positive in the diagnostic sample.

Processing and Shipping

Specimen Processing:Do not process. Store at room temperature.
Shipping Instructions:Ship at room temperature. Do not refrigerate or freeze.
Stability:Whole blood samples are stable for 5 days at room temperature. Do not refrigerate or freeze.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC-East Bank)


Reference Range:See interpretive report.
Use:Mutations in EZH2 are seen in the germinal center subtype of DLBCL, while mutations in CARD11, MYD88, and CD79B are seen in the activated B-cell subtype. Mutation or loss of TP53 are associated with worse survival.

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