FLT3 Mutation Detection ITD/TKD by NGS


Sunquest Code:FLT3CoPath Code:FLT3
  Epic Name:FLT3 Mutation Detection ITD/TKD by NGS
Sunquest Code (non-Epic sites):LAB8069  
Synonyms:D835; ITD; TKD
Methodology:Next generation sequencing and PCR/restriction digest
Turnaround Time:Performed Mon-Fri; results are reported within 10-14 days.


Collection Instructions

Specimen:Blood, bone marrow
Optimal Volume:10 mL blood; 3 mL bone marrow
Minimum\Peds Volume:3 mL blood; 1 mL bone marrow
Container:Yellow (ACD, Solution A) tube available from laboratory (Purple (EDTA))
Collection Instructions:Bone marrow clot acceptable for testing.
Causes for Rejection:Whole blood: frozen or clotted specimen, incorrect anticoagulant, specimen more than 5 days old. All testing will be cancelled if engraftment testing is 100% donor engrafted in consultation with clinician.
Shared blood or bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers.


Processing and Shipping

Specimen Processing:Do not process. Store at room temperature.
Shipping Instructions:Ship at room temperature. Do not refrigerate or freeze.
Stability:Whole blood samples are stable for 5 days at room temperature. Do not refrigerate or freeze.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC-East Bank)


Interpretive

Reference Range:See interpretive report.
Two reports are sent with this testing. The first report is sent at 3-5 days with results of PCR/digest assay, and the second report is sent at 10-14 days and includes the results of NGS assay.
Testing of FLT3 alone may be considered in follow-up of FLT3 mutated acute myeloid leukemia (AML); however, in these cases consider ordering the follow-up AML panel (LAB8047).


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