FLT3 Mutation Detection ITD/TKD by NGS
| Abbrev Code: | FLT3 | ||
| Order Code: | LAB8069 | Order Name: | FLT3 Mutation Detection ITD/TKD by NGS |
| Sunquest Code (non-Epic sites): | LAB8069 | ||
| Synonyms: | D835; ITD; TKD | ||
| Methodology: | Next generation sequencing and PCR/restriction digest | ||
| CPT Codes: | 81245 x1, 81246 x1, G0452 x1 | ||
| Turnaround Time: | Performed Mon-Fri; results are reported within 10-14 days. | ||
Collection Instructions
| Specimen: | Blood, bone marrow |
| Optimal Volume: | 10 mL blood; 3 mL bone marrow |
| Minimum\Peds Volume: | 3 mL blood; 1 mL bone marrow |
| Container: | Yellow (ACD, Solution A) tube available from laboratory Alternate Containers: Purple (EDTA) |
| Collection Instructions: | Bone marrow clot acceptable for testing. |
| Causes for Rejection: | Whole blood: frozen or clotted specimen, incorrect anticoagulant, specimen more than 5 days old. All testing will be cancelled if engraftment testing is 100% donor engrafted in consultation with clinician.
Shared blood or bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers. |
Processing and Shipping
| Specimen Processing: | Do not process. Store at room temperature. |
| Shipping Instructions: | Ship at room temperature. Do not refrigerate or freeze. |
| Stability: | Whole blood samples are stable for 5 days at room temperature. Do not refrigerate or freeze. |
| Test Performed at or Referral Lab | Molecular Diagnostics (UMMC-East Bank) |
Interpretive
| Reference Range: | See interpretive report.
Two reports are sent with this testing. The first report is sent at 3-5 days with results of PCR/digest assay, and the second report is sent at 10-14 days and includes the results of NGS assay. Testing of FLT3 alone may be considered in follow-up of FLT3 mutated acute myeloid leukemia (AML); however, in these cases consider ordering the follow-up AML panel (LAB8047). |
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