NPM1 Mutation Detection by NGS
| Sunquest Code: | NPM1SG | CoPath Code: | NPM1SG |
| Epic Code: | LAB8070 | Epic Name: | NPM1 Mutation Detection by NGS |
| Methodology: | Next generation sequencing | ||
| Turnaround Time: | Performed Mon-Fri; results are reported within 10-14 days. | ||
Collection Instructions
| Specimen: | Blood, bone marrow |
| Optimal Volume: | 10 mL blood; 3 mL bone marrow |
| Minimum\Peds Volume: | 3 mL blood, 1 mL bone marrow |
| Container: | Yellow (ACD, Solution A) tube available from laboratory (Purple (EDTA)) |
| Collection Instructions: | Bone marrow clot acceptable for testing. |
| Causes for Rejection: | Whole blood: frozen or clotted specimen, incorrect anticoagulant, specimen more than 5 days old. All testing will be cancelled if engraftment testing is 100% donor engrafted in consultation with clinician.
Shared blood or bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers. The diagnostic sample for hematology panels (not including MPN) requires at least 20% blasts to perform testing. Follow-up samples for the hematology panels will be tested for all gene(s) positive in the diagnostic sample. |
Processing and Shipping
| Specimen Processing: | Do not process. Store at room temperature. |
| Shipping Instructions: | Ship at room temperature. Do not refrigerate or freeze. |
| Stability: | Whole blood samples are stable for 5 days at room temperature. Do not refrigerate or freeze. |
| Test Performed at or Referral Lab | Molecular Diagnostics (UMMC-East Bank) |
Interpretive
| Reference Range: | See interpretive report. |
| Use: | Testing of NPM1 alone may be considered in follow-up of NPM1 mutated acute myeloid leukemia (AML); however in these cases consider ordering the Follow-Up AML Panel (LAB8047). |
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