MPL, CALR NGS Reflex Panel

Abbrev Code:MPLCAL   
Order Code:LAB8109Order Name:MPL CALR NGS Reflex Panel
Synonyms:MPN; JAK2
Methodology:Next generation sequencing
CPT Codes: 81219 x1, 81403 x1, G0452 x1
Turnaround Time:Performed Mon-Fri; results are reported within 7-10 days.

Collection Instructions

Specimen:Blood, bone marrow
Optimal Volume:10 mL blood; 3 mL bone marrow
Minimum\Peds Volume:3 mL blood; 1 mL bone marrow
Container:Yellow (ACD, Solution A) tube available from laboratory
Alternate Containers: Purple (EDTA)
Collection Instructions:Bone marrow clot acceptable for testing.
Causes for Rejection:Frozen or clotted specimen; incorrect anticoagulant; specimen more than 5 days old.
Shared blood and bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers.

Processing and Shipping

Specimen Processing:Do not process. Store at room temperature.
Shipping Instructions:Ship at room temperature. Do not refrigerate or freeze.
Stability:Whole blood samples are stable for 5 days at room temperature. Do not refrigerate or freeze.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC East Bank)


Reference Range:See interpretive report.
Use:JAK2 with reflex testing should be ordered if there is high clinical suspicion for a myeloproliferative neoplasm in a non-urgent clinical context. When this test is ordered, if a JAK2 mutation is not detected, reflex testing will be performed for CALR and MPL. There may be a delay in reporting CALR and MPL results if this is required. JAK2 mutations are present in almost all polycythemia vera (PV) patients; however, JAK2 mutations are present in only 60-65% of both essential thrombocythemia (ET) and primary myelofibrosis (MF) patients. CALR mutations are present in 20-25% of both ET and MF; while MPL mutations are present in approximately 3% and 7% in ET and MF patients respectively.

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