MPL, CALR NGS Reflex Panel
| Sunquest Code: | MPLCAL | CoPath Code: | MPLCALR |
| Epic Code: | LAB8109 | Epic Name: | MPL CALR NGS Reflex Panel |
| Synonyms: | MPN; JAK2 | ||
| Methodology: | Next generation sequencing | ||
| Turnaround Time: | Performed Mon-Fri; results are reported within 7-10 days. | ||
Collection Instructions
| Specimen: | Blood, bone marrow |
| Optimal Volume: | 10 mL blood; 3 mL bone marrow |
| Minimum\Peds Volume: | 3 mL blood; 1 mL bone marrow |
| Container: | Yellow (ACD, Solution A) tube available from laboratory (Purple (EDTA)) |
| Collection Instructions: | Bone marrow clot acceptable for testing. |
| Causes for Rejection: | Frozen or clotted specimen; incorrect anticoagulant; specimen more than 5 days old.
Shared blood and bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers. |
Processing and Shipping
| Specimen Processing: | Do not process. Store at room temperature. |
| Shipping Instructions: | Ship at room temperature. Do not refrigerate or freeze. |
| Stability: | Whole blood samples are stable for 5 days at room temperature. Do not refrigerate or freeze. |
| Test Performed at or Referral Lab | Molecular Diagnostics (UMMC-East Bank) |
Interpretive
| Reference Range: | See interpretive report. |
| Use: | JAK2 with reflex testing should be ordered if there is high clinical suspicion for a myeloproliferative neoplasm in a non-urgent clinical context. When this test is ordered, if a JAK2 mutation is not detected, reflex testing will be performed for CALR and MPL. There may be a delay in reporting CALR and MPL results if this is required. JAK2 mutations are present in almost all polycythemia vera (PV) patients; however, JAK2 mutations are present in only 60-65% of both essential thrombocythemia (ET) and primary myelofibrosis (MF) patients. CALR mutations are present in 20-25% of both ET and MF; while MPL mutations are present in approximately 3% and 7% in ET and MF patients respectively. |
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