Children's Oncology Group (COG) Flow Cytometry Bone Marrow
| Abbrev Code: | COGBLD | ||
| Order Code: | LAB560 | Order Name: | COG Flow Cytometry Bone Marrow |
| Order Instructions: | Appropriate for patients <20 years of age only. | ||
| Synonyms: | MRD | ||
| Methodology: | Multi-color immunofluorescence and flow cytometry | ||
| CPT Codes: | 88184 x1, 88185 x5, 88187 x1, 88188 x1, 88189 x1 | ||
| Turnaround Time: | Performed Mon-Fri 08:00-17:00 and on Saturday before noon. Results are reported within 1-3 business days. | ||
| Special Instructions: | Provide diagnosis, age, collection date, and time. Samples will be tested Mon-Fri 08:00-17:00 and on Saturday before noon. Samples are received in the Core lab when the Flow Cytometry lab is not staffed. Contact the IFC laboratory at 612-273-5248 for further information. If testing is needed outside of scheduled hours, contact the hematopathology fellow. | ||
Collection Instructions
| Specimen: | Bone marrow |
| Optimal Volume: | 4 mL |
| Minimum\Peds Volume: | 2 mL |
| Container: | Syringe, sterile, no needle Alternate Containers: Green (sodium heparin, no gel), Purple (EDTA) |
| Collection Instructions: | Collect in a sterile syringe with 0.5-1.0 mL of 1000 unit heparin per 5 mL of bone marrow to prevent clotting. Alternately collect in a sterile syringe and transfer immediately to a green (sodium heparin, no gel) or purple (EDTA) tube. |
| Causes for Rejection: | Improper specimen collection or handling; clotted specimen; specimen received >48 hours after collection. |
Processing and Shipping
| Specimen Processing: | Do not process. Store in refrigerator. |
| Shipping Instructions: | Ship at room temperature. |
| Stability: | 48 hours at room temperature or refrigerated. |
| Test Performed at or Referral Lab | UM Flow Cytometry (W) (UMMC East Bank) |
Interpretive
| Reference Range: | By report. |
| Use: | Use this test code for patients enrolled in COG B-ALL trials requiring decentralized flow MRD testing. COG trials are appropriate for patients <20 years old, and can only be ordered while logged in as a UR context. This test code is for day 29 bone marrow specimens. Please contact the Flow Cytometry laboratory at 612-273-5248 with questions. |
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