Aerobic Bacterial Culture Routine


Abbrev Code:AERCX, AERCXSTN   
Order Code:LAB500Order Name:Aerobic Bacterial Culture Routine
Order Instructions:To include a Gram stain with culture, select "with Gram Stain" at the time of order.
Synonyms:Amniotic; Bone; Abscess; Body Fluid; Contact Lens; Catheter Tip; CSF; Cyst; Endometrium; Eye; Foreign Body; Gastric; Aspirate; Genital; Perirectal; Semen; Skin; Tissue; Urethral; Prostatic; Wound; Culture
Methodology:Culture, Aerobic
CPT Codes: 87205 x1, 87070 x1, 87070 x1, 87070 x1, 87070 x1, 87070 x1, 87070 x1, 87070 x1, 87070 x1, 87070 x1, 87070 x1, 87070 x1, 87070 x1, 87070 x1, 87077 x1
Test Includes:Routine Culture for Aerobic Bacteria only
Turnaround Time:Performed 24 hours/day; preliminary results are reported within 24 hours; final results are reported within 7 days.
Associated Links:

IDDL (Microbiology/Virology) Collection Container Guidelines


Collection Instructions

Collection Instructions:
Specimen Type Instructions Volume Container Alternate Container
Abscess Prepare surface with povidone iodine solution; let dry.
Aspirate fluid/purulent material with syringe. Expel air bubbles from syringe; inject sample into sterile container
Deliver to laboratory immediately.		 Optimal: 5 mL
Minimum: 1 mL		 Sterile Container
Eswab – for use only if fluid/material cannot be aspirated.
 
 
Aspirate
Cyst
Body Fluid:
Amniotic, Aqueous, Ascites, Gastric, Ocular, Pericardial, Pleural, Peritoneal, Synovial, Vitreous, Milk 
Semen Collect in sterile container
Washing
Bone Marrow Cultures should be taken after specimens for cell morphology studies, genetic studies, cell marker studies and other cell studies. Use a new syringe for each. Clean top of anticoagulant tube with alcohol wipe before injecting. Optimal: 4-8 mL
Minimum: 1.5 mL		 SPS
Pediatric Blood Culture Bottle
Brushing Skin: Cleanse with 70% alcohol. Scrape off skin at active border of lesion; include some health skin. Place Brush in sterile container. N/A Brush in Sterile Container
N/A
CSF Wear gloves, gown and mask. Prepare skin as for blood culture. Drape surrounding skin with sterile linen. Separate tubes should be collected for 1) bacteriology, 2) heme, & 3) chem. Deliver to lab immediately. Send unprocessed speciment (tube 3 or 4, or tube 2 of 3) to IDDL. Optimal: 2mL
Minimum: 0.3 mL		 CSF Container
N/A
Bone:
Biopsy, Core Biopsy, Curetting, Fracture, Fragments, Resection, Exostosis, Osteophyte, Resection 		Place specimen in sterile leakproof container. Visible amount of specimen. Sterile Container
 Eswab – for use only if fluid/material cannot be aspirated.
 
Calculus/Stone
Foreign Body
Tissue:
Biopsy, Fine needle aspiration
Placenta Place patient in lithotomy position, insert speculum, and visualize cervical os.
Remove mucous plug, wipe cervical os with povidone iodine solution. Wipe off povidone iodine solution.
Aspirate material through the sleeve of a rush or curette sampling implement to avoid normal flora contamination.
Place the aspirate in a sterile tube.
Do not collect on swabs to prevent contamination with normal cervical/vaginal flora and to ensure adequate material is collected. Specimen can be swabbed after removal if shared with Surgical Pathology.
Anaerobic container can be used for aerobic and anaerobic culture.		 Visible amount of specimen. Sterile Container
 Eswab – for use only if shared with surgical pathology.
 
Swab Clean Surface with sterile sponge and sterile water.
If purulent material is available to be Aspirated collect in Sterile container for improved sensitivity. 		Eswab- N/A
Sterile Container:
Optimal: 5 mL
Minimum: 0.5 mL 		Sterile Container
 Eswab – for use only if fluid/material cannot be aspirated.
 
Skin
Wound
Vaginal Fluid Avoid contamination with normal flora from skin, rectum, vaginal tract or other body surface. N/A Eswab
N/A


Processing and Shipping

Specimen Processing:Store at room temperature.
Shipping Instructions:Ship at room temperature. Must arrive within 24 hours.

CSF must be packaged in a RED STAT bag and transported to the testing laboratory immediately.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory  (Grand Itasca, Range, UMMC East Bank)


Interpretive

Reference Range:No growth
Use:Isolate and identify pathogenic organisms.


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