Muscle Biopsy Routine
| Abbrev Code: | XMISC | ||
| Order Code: | LAB4909 | Order Name: | Laboratory Miscellaneous Order |
| Order Instructions: | Call Neuromuscular Lab at least 24 Hr before proposed procedure at 612-209-9401 (Admin) or 952-994-2804 (Tech) BEFORE scheduling and on day of procedure to verify timing of procedure and to ensure staff availability/questions. | ||
| Methodology: | Light microscopy and histochemistry on unfixed tissue. | ||
| CPT Codes: | 88314 x5, 88319 x12 | ||
| Test Includes: | H&E, PAS, Oil-Red-O, Congo red, Trichrome, ATPase (pH 4.35-4.65 and 9.4) Metachromatic ATPase, NADH-TR Succinic Acid Dehydrogenase (SDH), Modified SDH, Cytochrome Oxidase (COX), Alpha Glycerophosphate (aGP), Fast Blue B, Phosphorylase A, Myoadenylate Deaminase (MAD) and Non-specific esterase. | ||
| Turnaround Time: | Results are reported within 2 weeks. | ||
| Associated Links: | |||
Collection Instructions
| Specimen: | Tissue |
| Optimal Volume: | 2 pieces of tissue 1 cm by 0.5 cm |
| Container: | Container, Tissue, sterile |
| Collection Instructions: | Provide two separate pieces of muscle tissue. Adult muscle specimens should be ideally at least 1 cm long and 0.5 cm in diameter; for pediatric patients the sample size may vary. The muscle itself should not be exposed to local anesthetics, which can cause artifact changes when processing.
Wrap both pieces of fresh muscle in 4x4 saline moistened guaze (squeeze out excess) and place in an appropriately labeled specimen cup. On label, document what muscle was excised, and time sample was placed in container and attach to cup. |
Processing and Shipping
| Specimen Processing: | Do not process. Do not store. |
| Shipping Instructions: | Biopsy performed on East Bank OR: Send specimen immediately to the East Core Lab Sendouts Dept. Please ship specimen in a styrofoam container containing WET ICE. East Core Lab Sendouts will notify the rerefernce lab personnel about upcoming delivery via phone call to 612-209-9401 (Hope-Admin) or 952-994-2804 (Jackie-Tech). See address below.
Biopsy performed at CSC: Send specimen STAT via courier services to the Neuromuscular Lab within one hour of collection. Please ship specimen in styrofoam container containing WET ICE. CSC Sendouts will notify the reference lab personnel about upcoming delivery via phone call to 612-209-9401 (Hope-Admin) or 952-994-2804 (Jackie-Tech). See address below. Biopsy performed at West Bank OR: Send specimen STAT via courier services to neuromuscular lab within one hour of collection. Please ship specimen in a biohazard marked sytrofoam container containing WET ICE. West Core Lab Sendouts will notify the reference lab personnel about upcoming delivery via phone call to 612-209-9401 (Hope-Admin) or 952-994-2804 (Jackie-Tech). See address below. Specimen must arrive at the U of MN Neuromiuscuilar Lab within 1 hour of collection between 8:00 am-2:00 pm. If unable to reach the lab personnel, please page Dr. Karachunski at 612-899-7635. Moose Tower, 13th Floor, Room 13-250, Attention: Hope Amundsen, 515 Delaware Street SE, Minneapolis, MN 55455 |
| Test Performed at or Referral Lab | Lab Sendouts (U of MN Neuromuscular Pathology Lab) |
Interpretive
| Reference Range: | By report. |
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