HCV Quant PCR Confirmatory Reflex to Genotyping


Abbrev Code:HCVPCRRFX   
Order Code:LAB6673Order Name:HCV Quant PCR Confirmatory Reflex to Genotyping
Methodology:Real-Time polymerase chain reaction (PCR)
CPT Codes: 87522 x1, 87522 x1, 87902 x1, 87902 x1
Turnaround Time:Performed twice weekly; results are reported within 4 days. HCV Quant results greater than 5.0 log are sent to the reference lab for genotyping Sun-Sat and reported within 5-8 days.
Associated Links:

Hepatitis C Screening with Reflex Cascade Algorithm



Collection Instructions

Specimen:Blood
Optimal Volume:8 mL
Minimum\Peds Volume:4 mL
Container:Red or gold (gel)
Alternate Containers: 10 mL Purple EDTA tube
Collection Instructions:Serum is preferred. EDTA plasma is also acceptable. Serum is the only acceptable sample for neonates.
Causes for Rejection:Heparinized specimens and sources other than purple EDTA plasma, red or gold gel serum are not acceptable for testing.


Processing and Shipping

Specimen Processing:Centrifuge within 24 hours of collection and aliquot 4 mL, 1.8 mL minimum, plasma or serum. Store in refrigerator up to 3 days. Freeze samples if transport time is greater than 3 days.
Shipping Instructions:Ship at refrigerated or frozen temperature.
Stability:3 days at refrigeratred temperature; 40 days frozen (-20 to -80°C).
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory   (UMMC East Bank)


Interpretive

Reference Range:Not detected.
The HIV Quant assay has a reportable range of 15 to 100,000,000 IU/mL (1.18 log to 8.0 log IU/mL) for quantification of HCV RNA in plasma or serum.
 
Result Indication
Not Detected The assay was unable to detect HCV RNA within the specimen
<15 IU/mL
(<1.18 log IU/mL)
HCV RNA is detected but the level present is less than the lower quantification limit of the assay
>100,000,000 IU/mL
(>8.0 log IU/mL)
HCV RNA is detected, but the level present is above the upper quantification limit of the assay
Use:Used for patients born between 1945-1965, following the CDC recommendations with an HCV reactive antibody. Can also be used for any patient for which it is clinically recommended.


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