Abbrev Code:GENT   
Order Code:LAB28Order Name:Gentamicin Level
Methodology:Roche: CEDIA homogenous enzyme immunoassay
Vista: Turbidmetric rate
CPT Codes: 80170 x1, 80170 x1
Turnaround Time:Performed and reported 24 hours/day.
Special Instructions:Comment last dose information on request. Beta-lactam antibiotics and cephalosporins will inactivate aminoglycosides causing falsely low results.
Associated Links:

Roche Go Live Schedule

Collection Instructions

Optimal Volume:2.5 mL
Minimum\Peds Volume:0.6 mL; pediatric patients only: 0.4 mL in green (lithium heparin, no gel)
Collection Instructions:Chemistry Tests Containers Chart
Trough levels: Collect a minimum of 6 hours after last dose; recommend collection immediately prior to the next dose. Peak levels: collect 60 minutes after the end of IV infusion; 1-2 hours if decreased renal function.
Causes for Rejection:Processed >2 hours after collection and patient is receiving beta-lactam antibiotics structurally similar to gentamicin (e.g. netilmicin, sagamicin and sisomicin) or cephalosporin; collection in a gel tube

Processing and Shipping

Specimen Processing:Centrifuge and assay immediately. If testing is delayed more than 2 hours, then aliquot and freeze the sample, 1.25 mL, 0.2 mL minimum (adult) 0.1 mL minimum (peds),

If specimen is received more than 2 hours after collection, check with the PCU/Clinic to see whether the patient is receiving beta-lactam antibiotics or cephalosporins; if so, cancel the test. Beta-lactam antibiotics and cephalosporins will inactivate aminoglycosides causing falsely low results.
Shipping Instructions:Ship at refrigerated temperature.
Stability:Roche: 7 days refrigerated; 4 weeks frozen.
Test Performed at or Referral Lab Chemistry  (Roche-Grand Itasca, Roche-Lakes, Roche-Range, Roche-Ridges, Roche-Southdale, Roche-St. John's, Roche-UMMC East, UMMC/UMMCH-West Bank)


Critical Range:
Site Trough  Peak 
Vista   >16 mg/L
Roche   >16 ug/mL
mg/L = ug/mL
Therapeutic Range:
Site Trough  Peak  Random 
Vista 1-2 mg/L 5-8 mg/L  
Roche 1-2 ug/mL 5-8 ug/mL  
mg/L = ug/mL
Use:Monitor therapeutic dosing and evaluate toxicity. Half-life is 1.5-3 h (adult less than 30 y); 3-6 h (adult 30 y or greater); 4-8 h (neonate).

Click HERE to Report test errors or omissions.
*If no email program is associated with this computer, please contact: for TestID: 698"