Abbrev Code:GENT   
Order Code:LAB28Order Name:Gentamicin Level
Methodology:CEDIA homogenous enzyme immunoassay
CPT Codes: 80170 x1, 80170 x1
Turnaround Time:Performed and reported 24 hours/day.
Special Instructions:Comment last dose information on request. Beta-lactam antibiotics and cephalosporins will inactivate aminoglycosides causing falsely low results.

Collection Instructions

Optimal Volume:2.5 mL
Minimum\Peds Volume:0.6 mL
Container:Red (no gel)
Collection Instructions:Trough levels: Collect a minimum of 6 hours after last dose; recommend collection immediately prior to the next dose. Peak levels: collect 60 minutes after the end of IV infusion; 1-2 hours if decreased renal function.
Causes for Rejection:Processed >2 hours after collection and patient is receiving beta-lactam antibiotics structurally similar to gentamicin (e.g. netilmicin, sagamicin and sisomicin) or cephalosporin; collection in a gel tube

Processing and Shipping

Specimen Processing:Centrifuge and assay immediately. If testing is delayed more than 2 hours, then aliquot and freeze the sample, 1.25 mL, 0.2 mL minimum (adult) 0.1 mL minimum (peds),

If specimen is received more than 2 hours after collection, check with the PCU/Clinic to see whether the patient is receiving beta-lactam antibiotics or cephalosporins; if so, cancel the test. Beta-lactam antibiotics and cephalosporins will inactivate aminoglycosides causing falsely low results.
Shipping Instructions:Ship at refrigerated temperature.
Stability:7 days refrigerated; 4 weeks frozen.
Test Performed at or Referral Lab Chemistry  (Roche-Grand Itasca, Roche-Lakes, Roche-Range, Roche-Ridges, Roche-Southdale, Roche-St. John's, Roche-UMMC East, Roche-UMMC West)


Critical Range:Peak: >16 ug/mL
mg/L = ug/mL
Therapeutic Range:Trough: 1-2 ug/mL
Peak: 5-8 ug/mL
mg/L = ug/mL
Use:Monitor therapeutic dosing and evaluate toxicity. Half-life is 1.5-3 h (adult less than 30 y); 3-6 h (adult 30 y or greater); 4-8 h (neonate).

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