Gentamicin
| Abbrev Code: | GENT | ||
| Order Code: | LAB28 | Order Name: | Gentamicin Level |
| Methodology: | Grand Itasca: Homogenous enzyme immunoassay
St Joseph: Chemiluminscent microparticle immunoassay (CMIA) All other sites: Turbidmetric rate |
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| CPT Codes: | 80170 x1 | ||
| Turnaround Time: | Performed and reported 24 hours/day. | ||
| Special Instructions: | Comment last dose information on request form. | ||
Collection Instructions
| Specimen: | Blood |
| Optimal Volume: | 2.5 mL |
| Minimum\Peds Volume: | 0.6 mL; pediatric patients only: 0.4 mL in green (lithium heparin, no gel) |
| Container: | Red (no gel), St Joseph - Green (lithium heparin, no gel) Alternate Containers: Green (lithium heparin, no gel), St. Joseph - Red (no gel) |
| Collection Instructions: | Trough levels: Collect immediately prior to the next dose. Peak levels: collect 60 minutes after the end of IV infusion; 1-2 hours if decreased renal function. |
| Causes for Rejection: | Processed >2 hours after collection and patient is receiving beta-lactam antibiotics structurally similar to gentamicin (e.g. netilmicin, sagamicin and sisomicin) or cephalosporin; collection in a gel tube |
Processing and Shipping
| Specimen Processing: | Centrifuge and assay immediately. If testing is delayed more than 2 hours, then aliquot and freeze the sample, 1.25 mL, 0.2 mL minimum (adult) 0.1 nL minimum (peds), |
| Shipping Instructions: | Ship on dry ice. |
| Stability: | If specimen is received more than 2 hours after collection, check with the PCU/Clinic to see whether the patient is receiving beta-lactam antibiotics or cephalosporins; if so, cancel the test. Beta-lactam antibiotics and cephalosporins will inactivate aminoglycosides causing falsely low results. |
| Test Performed at or Referral Lab | Chemistry (Range, UMMC-East Bank, Lakes, Ridges, Northland, Southdale, UMMC/UMMCH-West Bank, Grand Itasca, St. Joseph's) |
Interpretive
| Critical Range: | Grand Itasca: Trough: Greater than 2 ug/mL; Peak: Greater than 12 ug/mL
St Joseph: Greater than 16 mg/L All other sites:
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| Therapeutic Range: | Depending on dosage:
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| Use: | Monitor therapeutic dosing. Half-life is 1.5-3 h (adult <30 y); 3-6 h (adult >30 y); 4-8 h (neonate). |
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