BNP (B-type Natriuretic Peptide) Woodwinds Only


Abbrev Code:BNP   
Order Code:LAB1034Order Name:B-Type Natriuretic Peptide (Woodwinds Only)
Methodology:Architect: Chemiluminescence
CPT Codes: 83880 x1, 83880 x1
Turnaround Time:Performed and reported 24 hours/day.


Collection Instructions

Specimen:Blood
Optimal Volume:2.5 mL
Minimum\Peds Volume:0.6 mL
Container:Purple (EDTA)
Causes for Rejection:Severely hemolyzed specimens. If a specimen appears to be severely hemolyzed, another specimen should be obtained and tested.
Contraindications:Large doses of biotin (10 mg or more per day) may cause clinically significant interference in N-Terminal pro brain natriuretic peptide levels. If interference is suspected, it is strongly recommended that biotin is discontinued for at least one week prior to retesting.


Processing and Shipping

Specimen Processing:Centrifuge and aliquot 1.25 mL, 0.20 mL minimum. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature to arrive within 24 hours. Alternatively, ship aliquoted plasma specimens on dry ice.
Stability:Whole blood and plasma samples, stored at 2-8°C must be tested within 24 hours of collection. Whole blood and plasma samples, stored at room temperature must be tested within 4 hours of collection.

If samples cannot be tested within the given times for room temperature or 2-8°C storage, they may be separated by centrifugation and frozen for up to 3 months at -20°C or below in plastic tubes.
Test Performed at or Referral Lab Chemistry  (Woodwinds)


Interpretive

Reference Range:BNP Reference Ranges for Woodwinds
Limitations:
  1. EDTA plasma, collected in plastic tubes, should be used for this assay. The use of glass collection tubes, or other sample types, such as serum or plasma with other anticoagulants, is not recommended.
  2. For diagnostic purposes, the ARCHITECT BNP results should be used in conjunction with other clinical data, e.g., symptoms, medical history, etc. If BNP results are not consistent with other clinical observations, additional information may be required for diagnosis.
  3. Specimens from patients who have received preparations of mouse monoclonal antibodies for diagnosis or therapy may contain human anti-mouse antibodies (HAMA). Such specimens may show either falsely elevated or depressed values when tested with assay kits which employ mouse monoclonal antibodies. ARCHITECT BNP reagents contain a component that reduces the effect of HAMA reactive specimens. Additional clinical or diagnosis information may be required to determine patient status.
  4. Heterophilic antibodies in human plasma can react with reagent immunoglobulins, interferring with in vitro immunoassays. The presence of heterophilic antibodies in a patient may cause anomalous values to be observed.
  5. ARCHITECT BNP results should not be used interchangeably with other manufacturers' methods for BNP or NT-proBNP determinations.
  6. Measurements of BNP should occur prior to Nesiritide (Natrecor), recombinant BNP treatment and 2 hours post-treatment.
Use:B-Type Natriuretic Peptide (BNP) protein is classed as a cardiac neurohormone and originates from the myocytes of the atrium and ventricles of the heart. It is secreted under conditions of myocardial stretching, volume overload, and increased ventricular filling pressures. ProBNP, the immediate precursor of BNP cleaves to form BNP and NT-proBNP in a 1:1 ratio. NT-proBNP concentrations are higher because the half-life is one to two hours, compared to 22 minutes for BNP. NT-proBNP is used to differentiate heart failure from pulmonary disease in a patient presenting with symptoms of dyspnea and shortness of breath. Marked elevations of NT-proBNP may be observed in states other than left ventricular congestive heart failure, including: acute coronary syndromes, right heart strain/failure, as well as advanced age. Falsely low NT-proBNP in congestive heart failure patients may be observed with increasing body mass index.


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