Phospholipase A2 Receptor, Immunofluorescence


Abbrev Code:MMMISC   
Order Code:LAB4909Order Name:Laboratory Miscellaneous Order
Order Instructions:Can be used in conjunction with a quantitative ELISA (LAB8270) to confirm a positive result, especially for a low-level titer. Since the IFA is more sensitive, it can also be used to detect/follow patients with low level antibodies not detected by ELISA.
Synonyms:PLA2R; Anti-PLA2R
Methodology:Indirect Immunofluorescence Assay (IFA)
CPT Codes: 86255 x1
Turnaround Time:Specimens sent to reference laboratory Monday-Friday; results are reported within 8 days.
Compliance:

This test was developed and its performance characteristics determined by Laboratory Medicine and Pathology, Mayo Clinic. This test has not been cleared by the U.S. Food and Drug Administration.



Collection Instructions

Specimen:Blood
Optimal Volume:3 mL
Minimum\Peds Volume:1.2 mL
Container:Red (gel)
Alternate Containers: Red (no gel)
Collection Instructions:Deliver to lab as soon as possible to allow for required processing within 2 hours of collection.
Causes for Rejection:Gross hemolysis


Processing and Shipping

Specimen Processing:Centrifuge and aliquot a minimum of 0.5 mL serum within 2 hours of collection. Store at refrigerated temperature.
Shipping Instructions:Ship at refrigerated temperature.
Stability:8 hours at room temperature; 14 days at refrigerated or frozen.
Test Performed at or Referral Lab (Mayo)
Referral Lab number:PLA2I


Interpretive

Reference Range:Negative
Limitations:Absence of circulating anti-phospholipase A2 receptor autoantibodies does not rule out a diagnosis of primary MN.
Use:Distinguishing primary from secondary membranous nephropathy in patients with low levels of anti-phospholipase A2 receptor (PLA2R) antibodies.
Screening for anti-PLA2R antibodies.
Monitoring patients with membranous nephropathy at very low antibody titers.


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