C. difficile Antigen and Toxins A/B by Enzyme Immunoassay


Abbrev Code:CDABTOX   
Order Code:PLAB8261Order Name:C. difficile Antigen and Toxins A/B by Enzyme Immunoassay
Order Instructions:Test performed by reflex from LAB8261 (CDBPCR) when sample is positive by PCR. This test is not orderable.
Methodology:Enzyme Immunoassay
CPT Codes: 87324 x1, 87324 x1
Turnaround Time:Performed daily; reported within 24 hours
Associated Links:

C. difficile Reflex Testing Results Information and Interpretation



Collection Instructions

Specimen:Feces, liquid/pourable/unformed/conforms to shape of container
Optimal Volume:3 mL
Minimum\Peds Volume:1 mL
Container:
Alternate Containers: Plastic leakproof container, sterile
Causes for Rejection:Specimens in formalin-based or alcohol-based fixative; Rectal swabs
Contraindications:Per manufacturer's package insert: No data exists on the effects of colonic washes, barium enemas, laxatives or bowel preparations on the performance of the C. DIFF QUIK CHEK COMPLETE test. All of these procedures can result in extensive dilution or the presence of additives tha may affect test performance.


Processing and Shipping

Specimen Processing:Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature <24 hours. Ship frozen >24 hours. Must arrive within 3 days.
Stability:24 hours at refrigerated temperature; 3 days frozen.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory   (UMMC East Bank)


Interpretive

Limitations:Per manufacturer's package insert: No data exists on the effects of colonic washes, barium enemas, laxatives or bowel preparations on the performance of the C. DIFF QUIK CHEK COMPLETE test. All of these procedures can result in extensive dilution or the presence of additives tha may affect test performance.
Use:C. difficile Toxin B PCR utilizes real-time PCR to detect toxin B gene sequences associated with toxin producing C. difficile in stool specimens collected from patients suspected of having Clostridioides difficile infection. The C. difficile enzyme immunoassay detects a C. difficile antigen, glutamate dehydrogenase (GDH), as a screen for the presence of C. difficile. It also confirms the presence of toxigenic C. difficile by detecting toxins A and B in fecal specimens from persons suspected of having C. difficile infection.  These tests are used to aid in the diagnosis of C. difficile disease in patients experiencing loose, watery stools with risk factors (e.g. recent antibiotic use, immunosuppression, hospital stay) and should be used in conjunction with patient history and clinical findings.


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