CD20 on B Cells, Blood

Abbrev Code:CD20B   
Order Code:LAB1407Order Name:CD20 on B Cells, Blood
Methodology:Flow Cytometry
CPT Codes: 86355 x1, 86356 x1
Turnaround Time:Specimen sent to reference laboratory Mon-Sat; reported within 2-3 days.

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

Collection Instructions

Optimal Volume:3 mL
Minimum\Peds Volume:1 mL
Container:Purple (EDTA)
Causes for Rejection:Secondary/pour off tubes, refrigerated or frozen specimens, gross hemolysis, gross lipemia.

Processing and Shipping

Specimen Processing:Collect and package specimens as close to shipping time as possible. It is recommended that specimen arrive within 24 hours of collection. 
Send whole blood in original tube; do not aliquot. Store at room temperature.
Shipping Instructions:Ship at room temperature.
Stability:4 days at room temperature.
Test Performed at or Referral Lab Lab Sendouts  (Mayo)
Referral Lab number:CD20B


Reference Range:
  %CD19 B Cells CD19 Absolute %CD20 B Cells CD20 Absolute CD45 Absolute
19 y & older 4.6-22.1% 56.6-417.4 cells/mcL 5.0-22.3% 74.4-441.1 cells/mcL NA
18-55 y NA NA NA NA 0.99-3.15 thou/mcL
56 y & older NA NA NA NA 1.00-3.33 thou/mcL
Use:This is the correct test to order if specifically confirming the absence of B cells due to suspected primary humoral or combined immunodeficiency or evaluating for CD19 deficiency.

If desirous of only quantitatively measuring total CD19 or CD20+ B cells, order TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood or CD20B / CD20 on B Cells, Blood, respectively. Do not order the detailed analysis of B cell subsets for this purpose.

This test should not be ordered for a comprehensive evaluation of peripheral B-cell subsets. For evaluation of memory B-cell subsets, transitional B cells, mature and immature B cells, order IABCS / B-Cell Phenotyping Profile for Immunodeficiency and Immune Competence Assessment, Blood.

This test should not be used for evaluating presence of CD20 on malignant or nonmalignant B cells. The following test should be used instead, CEE20 / CD20 Cell Expression Evaluation, Varies.

Click HERE to Report test errors or omissions.
*If no email program is associated with this computer, please contact:
[email protected] for TestID: 7015"