CD20 on B Cells, Blood
| Abbrev Code: | CD20B | ||
| Order Code: | LAB1407 | Order Name: | CD20 on B Cells, Blood |
| Methodology: | Flow Cytometry | ||
| CPT Codes: | 86355 x1, 86356 x1 | ||
| Turnaround Time: | Specimen sent to reference laboratory Mon-Sat; reported within 2-3 days. | ||
| Compliance: | This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration. |
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Collection Instructions
| Specimen: | Blood |
| Optimal Volume: | 3 mL |
| Minimum\Peds Volume: | 1 mL |
| Container: | Purple (EDTA) |
| Causes for Rejection: | Secondary/pour off tubes, refrigerated or frozen specimens, gross hemolysis, gross lipemia. |
Processing and Shipping
| Specimen Processing: | Collect and package specimens as close to shipping time as possible. It is recommended that specimen arrive within 24 hours of collection.
Send whole blood in original tube; do not aliquot. Store at room temperature. |
| Shipping Instructions: | Ship at room temperature. |
| Stability: | 4 days at room temperature. |
| Test Performed at or Referral Lab | Lab Sendouts (Mayo) |
| Referral Lab number: | CD20B |
Interpretive
| Reference Range: |
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| Use: | This is the correct test to order if specifically confirming the absence of B cells due to suspected primary humoral or combined immunodeficiency or evaluating for CD19 deficiency.
If desirous of only quantitatively measuring total CD19 or CD20+ B cells, order TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood or CD20B / CD20 on B Cells, Blood, respectively. Do not order the detailed analysis of B cell subsets for this purpose. This test should not be ordered for a comprehensive evaluation of peripheral B-cell subsets. For evaluation of memory B-cell subsets, transitional B cells, mature and immature B cells, order IABCS / B-Cell Phenotyping Profile for Immunodeficiency and Immune Competence Assessment, Blood. This test should not be used for evaluating presence of CD20 on malignant or nonmalignant B cells. The following test should be used instead, CEE20 / CD20 Cell Expression Evaluation, Varies. |
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