Platelet Antigen Genotyping Panel

Sunquest Code:PAGENO  
Epic Code:LAB4308Epic Name:Platelet Antigen Typing I
Synonyms:Platelet Antigen Genotyping, Level 1
Methodology:PCR and fluorescent hydrolysis probes
Test Includes:HPA-1, 2, 3, 4, 5,6, 9 and 15
Turnaround Time:Specimens are sent to reference laboratory Mon-Fri; results are reported within 7 days.
Special Instructions:A signed informed consent in the patient's medical record is required; the consent should not be sent to the laboratory. The link to the Genetic Testing Consent Form is provided as a convenience for the providers and genetic counselors.
Associated Links:

Genetic Testing Consent Form

Collection Instructions

Specimen:Blood, amniotic fluid, cord blood or cultured amniocytes
Optimal Volume:5 mL blood; 1 mL cord blood; 15 mL amniotic fluid, or 5 x 10 mg cultured amniocytes
Minimum\Peds Volume:3 mL blood (adult); 2 mL blood (pediatric); 7 mL amniotic fluid. Call laboratory for minimum of other specimens types (1-800-245-3117 ext. 6250)
Container:Purple (EDTA)
[Alternate Containers: Plastic leakproof container, sterile]
Collection Instructions:Use purple (EDTA) for blood or cord blood; plastic leakproof container for other specimens.

Processing and Shipping

Specimen Processing:All specimens: do not process. Store at room temperature.
Shipping Instructions:Ship at room temperature or refrigerated.
Test Performed at or Referral Lab Lab Sendouts  (Versiti)
Referral Lab number:5600


Reference Range:Interpretive report.
Limitations:New variant alleles that posses polymorphisms within the region targeted by the oligonucleotide primers may not be identified with these assays.
Use:Confirmation of Platelet Antibody Specificity: Genotyping can be used in PTP and MPR cases to confirm the specificity of platelet-specific antibodies present in the patient's serum. Genotyping is particularly helpful in confirming antibody specificity in cases involving low frequency or "new" platelet alloantigens. Samples with Low Platelet Counts: In contrast to standard serologic platelet typing tests, genotyping does not require that blood samples contain adequate numbers of platelets since it utilizes DNA isolated from leukocytes or other cells.

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