Methemoglobin


Sunquest Code:MHGB  
Epic Code:LAB4187Epic Name:Methemoglobin
Methodology:Spectrophotometric analysis of hemolysate
Turnaround Time:Performed and reported 24 hours/day.
Special Instructions:Sample is pH dependent. Methemoglobin may revert to hemoglobin on standing. Ideally, test is performed within 2 hours of collection; up to 10% decrease may occur after 4 hours, up to a 16% decrease after 8 hours.


Collection Instructions

Specimen:Blood (arterial, venous, capillary)
Optimal Volume:1 mL
Minimum\Peds Volume:0.5 mL (3 mL syringe); 0.2 mL (tuberculin syringe); 0.1 mL (capillary tube)
Container:St Johns/St Joseph/Woodwinds - Dry heparin syringe, Dry heparin syringe
[Alternate Containers: Capillary (lithium heparin) gas tube, Green (lithium heparin, no gel), Green (lithium heparin, no gel) on ICE, Green (sodium heparin, no gel), Green (sodium heparin, no gel) on ICE, St Johns/St Joseph/Woodwinds-Green (lithium heparin, no gel), St Johns/St Joseph/Woodwinds - Capillary (lithium heparin) gas tube]
Collection Instructions:Sample should be free of air bubbles. If collecting in green (heparin) tube, do not remove stopper. Deliver to the laboratory immediately; if received within 20 minutes, ice is not required.
Causes for Rejection:Gross lipemia; presence of >1% sulfhemoglobin, >60 mg/L methylene blue or citrate.


Processing and Shipping

Specimen Processing:Do not store.
Shipping Instructions:Ship on wet ice to arrive within 8 hours, preferably 2 hours.
Test Performed at or Referral Lab Chemistry  (Range, UMMC-East Bank, Ridges, Northland, Southdale, UMMC/UMMCH-West Bank, UMMC NICU, M Health Clinic and Surgery Center, Grand Itasca, St. Joseph's, St. John's, Woodwinds)


Interpretive

Critical Range:Greater than 35%
Reference Range:0-3%; smokers may be slightly higher than nonsmokers.


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