DNA Marker Engraftment, Graft vs Host


Abbrev Code:GVHMD   
Order Code:LAB4726Order Name:DNA Marker Engraftment Graft vs Host
Synonyms:Maternal Engraftment; Donor Engraftment involved in GVH Disease; Third Party Engraftment; Engraftment, Other; Molecular
Methodology:Amplification of DNA and capillary electrophoresis
CPT Codes: 81265 x1, G0452 x1
Test Includes:BMT DNA Processing; DNA Marker-Polymorphism; DNA Marker-Engraftment.
Turnaround Time:Performed Mon-Fri; results are reported within 7-14 days, depending upon polymorphisms found.
Special Instructions:

Please call the Molecular Diagnostics Lab before sending sample.

Maternal engraftment patients

Identify patient's name, mother's name, and sex of patient. Indicate "maternal engraftment", "donor engraftment", or "third party engraftment" on request form. "Donor engraftment" and "third party engraftment" require recipient and donor pre-transplant sample, and recipient post-transplant (or post-transfusion) sample.

 

Graft versus Host Patients

For analysis to be completed, all three of the following sample types must be received:

·          Recipient cells (buccal swab)

·          Donor cells/DNA which have been previously banked (in HLA Lab or Cell Therapy) prior to transplant

·          Post-transplant material from patient

 

 



Collection Instructions

Specimen:Blood, bone marrow aspirate, bone marrow biopsy or tissue biopsy
Minimum\Peds Volume:Contact laboratory if unable to obtain specified collection volume
Container:Yellow (ACD, Solution A) tube available from laboratory
Alternate Containers: Purple (EDTA)
Collection Instructions:
Contact the Molecular Diagnostic Laboratory prior to specimen collection
  • Peripheral blood: Collect 10 mL in yellow ACD (Solution A) tube; alternate container is purple (EDTA) tube.
  • Bone marrow aspirate: Aspirate ACD (or EDTA) solution into syringe from open ACD (or EDTA) tube. Collect 5 mL bone marrow into syringe. Deliver syringe.
  • Tissue: 3 mm x 3 mm x 3 mm in sterile container. Deliver to laboratory within 1 hour, or freeze specimen at -70°C (liquid nitrogen or dry ice)
Causes for Rejection:Clotted specimen; necrotic tissue, incorrect anticoagulant. Add on testing to hematology samples is not acceptable. DNA extracted at non-CLIA certified (or equivalent) lab.
Contraindications:
Blood is fractionated into CD3+ if WBC is greater than 5 x 109/L and sample is <48 hours old. Bone marrow is not fractionated. Results are reported with the exact percentage of donor engraftment. Sensitivity +5% indicates the minimum amount of control DNA marker detectable for this specimen.    


Processing and Shipping

Specimen Processing:Store blood and bone marrow aspirate at room temperature. Store tissue at -70 degrees C. Contact laboratory prior to shipping.
Shipping Instructions:Ship blood and bone marrow at room temperature to arrive within 24 hours. Ship tissue at room temperature to arrive within 1 hour; alternatively, ship on dry ice to arrive within 24 hours.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC East Bank)
Referral Lab number:GVH


Interpretive

Reference Range:

Blood is fractionated into CD3+ and CD15+ if WBC is greater than 1.0 x 109/L and sample is less than 48 hours old. Bone marrow is not fractionated. Results are reported with the exact percentage of donor engraftment. “Sensitivity ±5%” indicates the minimum amount of control DNA marker detectable for this specimen.

 

When more than one of the ten STR markers is informative, the final result will be the average of all informative markers.

 



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